Determining the plasticity of CD34 expression

Previously, we established that OCI-AML-20 requires stroma to maintain CD34 expression (https://zenodo.org/record/1187083#.Wr5-1C7wbIV). In this experiment, we wanted to see whether those cells grown in suspension are able to regain CD34 expression when cells are reintroduced to stroma. We find that OCI-AML-20 regains CD34 expression when put back on to stroma (either MS5 or OP9). Full Read More …

Determining CD34 expression in the presence and absence of stroma

We are currently working on a manuscript to characterize one of the cell lines that we established from growing patient samples. I previously discussed how we established OCI-AML-20 and I’m currently working on some experiments to complete the manuscript. One of those experiments is determining whether the CD34 expression seen in OCI-AML-20 relies on the Read More …

Cloning an NSD3-Short-3xFLAG Construct into a Lentiviral Expression System

Antibodies are fundamental tools in the exploration of cellular biology. Unfortunately, there is a lack of high-quality NSD3 antibodies available. To overcome this, I have generated a bicistronic NSD3-Short-3xFLAG – IRES – PuroR lentiviral expression plasmid for the purpose of creating cell lines that stably express 3xFLAG-tagged NSD3-short. This will allow me to use a Read More …

Generation of fluorescently labelled OP9 stromal cells for growing patient leukemic cells

Our experiments use cells from patients with leukemia. These primary cells are notoriously difficult to culture, especially long term. To overcome this, we are using a co-culture system where we grow the leukemia cells with mouse stromal cells (OP9) to try to mimic some aspects of the bone marrow environment present in leukemia. Although essential Read More …

Project Overview: Testing polycomb repressive complex 2 (PRC2) inhibitors in acute myeloid leukemia

Acute myeloid leukemia or AML is a type of cancer that is the result of abnormal division and development of blood cells. AML has very poor outcomes, with 5-year survival of less than 50 percent and even less in patients over the age of 60 [1]. Within AML, there are many subgroups that can dictate Read More …

Project Overview: Understanding Enhancer Biology in Cancer

Every cell in your body contains the exact same set of genetic instructions encoded in its genome. However, for complex multicellular organisms such as ourselves, we depend on many specialized cell types that exhibit strikingly different features. This is largely accomplished by precisely controlling how and when genetic information is read out in each cell, Read More …